محمدرضا بختیاری author فرزانه عزیرمحسنی author افسانه رجبی author پونه خلیل‌زاده author سعید میردامادی author مسعود فلاح پور author مهران کیانی‌راد author text article 2004 per In this research, several soil and wastewater samples from Hexamine producing factory were cultured in complex and minimum medium containing hexamine. Twenty nine bacteria, 2 yeasts and 3 molds were isolated. Nine bacterial and 2 yeast strains grew in medium containing 10000 mg/l hexamine. The bacterial strains degraded more than 50% and yeast strains degraded 100% of Hexamine. After adaptation of strains, 12 strains of bacteria (Micrococcus and Pseudomonas strains), one yeast strain and two mold strains (Alternaria) degraded hexamin in medium containing 50000 mg / l hexamin. پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16764_83fa7fc00e84b2dbaeaaf0104313a930.pdf حوری سپهری author ساطین صالحی author یاسمن رسولی author text article 2004 per Recent studies have indicated that several plant extracts reputed to be lactogenic, are capable of secreting prolactin and growth hormone (GH). The compound which proved to be active in the plant extracts are derived from polysaccharides such as pectic acid and ?-glucan. The result of the present investigation showed that pectic acid and ?-glucan when added to the culture medium of the GH3/B6 rat pituitary cell line stimulates prolactin release. Pectic acid (100 ?g/ml) increased prolactin secretion after 30 minutes of incubation that is about 40% of control medium. ?-glucan showed stimulatory activity at the concentration of 10?g/ml after 48 hours and at higher concentration (50-100?g/ml) after 72 hours. Previous studies have shown that TRH (Thyrotropin Releasing Hormone) stimulated prolactin secretion in lactotrop cells. Therefore, TRH was used as a control to evaluate the capacity of the cells to respond to a secretory stimulus. پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16765_3bc81fd5adefbe0f2a9cf2e64344704f.pdf فریبا میقانی author حسن ابراهیم‌زاده author text article 2004 per Effect of different concentration of NaCl (0, 50, 100, 200 and 300 mM) at different growth and development satges (tillering, boot swellen, flowering and anthesis) in two wheat cultivars (Ghods: salt-sensitive, Boolani: salt-resistant) on concentration of foliar water soluble carbohydrates (WSC) and reducing carbohydrates (RC) was studied under greenhouse conditions. In general, in response to salinity treatments, concentration of WSC was higher in Boolani leaves than in Ghods. On the contrary, foliar RC level was higher in Ghods than in Boolani. Thus, it becomes clear that the role of WSC in osmotic regulation was more distinct in Boolani than in Ghods. Considering the more importance of WSC (compared to RC), in regulation of osmotic pressure, we may consider Boolani as a cultivar with more resistance to salinity. پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16766_bc1f4617d1ffd5c9cb29bcf3cd9e54a1.pdf محمد علی آموزگار author فریدون ملک زاده author text article 2004 per A moderately halophilic g+, rod shaped, spore-forming isolated from saline soil from Karaj, Iran, produced extracellular amylase when cultivated aerobically in medium containing starch, peptone, beef extract and NaCl. The maximum amylase production was secreted in the presence of 15% (w/v) Na2SO4. The isolate was capable of producing amylase in the presence of NaCl 10% (w/v), NaCH3COO 15% (w/v), or KCl 5% (w/v), with the results NaCl>NaCH3COO> KCl. Amylase production was not detected when ammonium nitrate, potassium nitrate, sodium nitrate or sodium citrate were used, although bacterial growth occurred. Potential of different carbohydrates in the amylase production was in the order: dextrin > starch > maltose > lactose > sucrose > glucose. Various carbon sources induced enzyme production. The pH, temperature and aeration optima for enzyme production were 7.8, 30°C and 200 rpm respectively, while the optimum pH and temperature for enzyme activity was 7.8 and 50°C respectively. The bacterium was g+, non–motile, oxidase ?, catalase +, aerobic Bacillus. Starch, casein, and gelatin were hydrolyzed. Nitrate and nitrite reduction were negative. The isolate grows well in media containing 0.5 to 24% (w/v) salt. Following the criteria of "Bergey’s Manual of Systematic Bacteriology", the isolate was identified as a Bacillus sp. strain MA. Further investigation is underway. پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16767_2b3c617b0d7e2224e7c9704da0468f4c.pdf هدایت‌ اله‌ قورچیان author نعمت‌اله‌ دوستی author text article 2004 per In this report the different methods of pulsed field gel electrophoresis and the parameters such as electrical field geometries, field angles, field strengths, type of buffer and its ionic strength, type of gel and its concentration, and the effect of temperature were considered. Among them a technique called "Contour Clamped Homogeneous Electric Field" was chosen for further studies. Based on this technique, a pulsed field gel electrophoresis system was designed and constructed. Finally, the ability of system for separation of large DNA molecules was examined. Using this system, we were able to separate the compositions of Saccharomyces serevisiae in the molecular weight range of 90 Kbp to 2.2 Mbp. پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16768_969272a2edb95b8593955c03947c7bb2.pdf ساطین صالحی author حوری سپهری author سیدشهاب الدین صدر author یاسمن رسولی author text article 2004 per One of the causes of infertility is antisperm antibodies (ASA) which are directed against sperm surface antigens. Alterations in the surface of sperm is one of factors inducing antisperm antibody formation in women. In this study, tray agglutination test (TAT) and indirect – mixed antiglobulin reaction test (indirect – MAR) were used on serum and cervical mucus (CM) of 117 infertile and 40 fertile women. The result shows that there is a significant correlation between two different methods (indirect– MAR and TAT), when were applied on serum (p< 0.0001, p< 0.0221) and on cervical mucus (p پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16769_77fc2d1b27184aa57d581a8146cbe2f8.pdf وحید لطفی گورچین قلعه author ناصر آق author حوری سپهری author text article 2004 per The effects of different salinities on the survival, growth, reproductive and lifespan characteristics of three Artemia populations from Urmia lake, Maharlu lake and small lagoons around Urmia lake were studied in laboratory conditions. Artemia cysts were collected from the above-mentioned lakes and lagoons and hatched according to the standard proceduer. Four replicates of 200 newly hatched nauplii were treated in salinities of 75, 100, 125, 150 and 175 ppt. The larvae were fed with algae Dunaliella teriolecta and chemically treated yeast known as Lansy PZ. Survival rate of the Artemia were calculated on days 8, 11, 14, 17, 20 and 23 of growth. Total lenghts also were measuerd on days 8, 11, 17, 20 and 23 of growth using a micrometer and were compared with each other. After attaining the adulthood, thirty pairs of adult Artemia (in case of bisexual spp.) and 30 adult female (in case of parthenogenetic populations) were transferred from all salinities into separate 50 ml falcon tubes to compare their reproductive and lifespan characteristics. The results showed that survival and growth rates decreased with increasing salinity. Reproductive characteristics such as reproduction frequency, total offsprings, number of offsprings in each reproduction and number of offsprings in each day of reproduction period reduced with increasing in salinity. Moreover, higher salinity prolonged the pre-reproductive period but shortened the total reproductive period. Higher salinities also had some impacts on the percentage of encystment, postreproductive period and life span, but existence of a logic relationships between salinity and these parameters could not be proved. پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16770_c0a30fcf39f01419e68f38697536e8ba.pdf حمزه امیری author دکتر حمید فهیمی author text article 2004 per The effect of different concentrations of NaCl and KCl on tissue culture of Phaseolus vulgaris L. has been examined. The obtained results showed that the increase NaCl and KCl concentrations has negative effects on the growth process of callus. In such a way that with increase of NaCl and KCl in the medium ,the index of callus, the fresh and dry weight and the ratio of as well as the amount of the total protein decrease while the amount of proline increased. These results also showed that the undesirable consequences of KCl is far more than that of NaCl in the similar concentrations. Therefore, KCl effects is the of the reverse the of effects of KNO3. The measurment of Na, K and Ca showed that when NaCl or KCl increases in the medium, the amount of Na and K in calluses will increase, respectively. There is also an antagonistic relationship between K and Na in lower concentrations. The increase of NaCl in the medium about 25 mM causes Ca increase in callus , there after, the increase of NaCl causes Ca decrease in callus.While the increase of KCl constantly decreases Ca. پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16771_51980dcf5077286715743fded1a7395f.pdf پیام بهزادی author ساسان رضایی author محمدرضا خرمی‌زاده author الهام بهزادی author مسعود امامی author text article 2004 per In this study, we tried to find out the morphological changes following the UVB-irradiation on these fungi and analyze the obtained results in molecular aspect. The following species of dermatophyte fungi (Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum and Microsporum canis) have been isolated from patients and selected for investigation in this project. For determining the possible molecular changes in dermatophytes due to UVB-irradiation (e.g. apoptosis feature), we next isolated high molecular weight DNA from non-irradiated as well as irradiated T. rubrum and compared them by gel-electrophoresis. The result of study indicated the inhibition of growth in irradiated colonies. The longer irradiation time caused slower rates of growth in these fungi. Besides, the changes in shape of colonies and pigmentation were observed in some cases. The microscopic studies of irradiated colonies indicate deformation of hyphae and changes in size and shape of macroconidia in all species. Besides, changes in number of microconidia in some species were determined. In the investigation of isolated DNAs from T. rubrum no differences in DNA banding patterns (apoptosis) was observed پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16772_96acc0d581527dc9efd53be041c34906.pdf اشرف الدین سخن‌سنج author حکمت علیخانی author مسعود میرمعصومی author text article 2004 per As one of basic requirements for resistance against enviromental stresses, plants produce and accumulate specific proteins inside the cells. Osmotin protein encoded by osmotin gene was reported to cause resistance to pathogens and environmental stresses such as salt-stress. Stress genetic engineered osmotin gene which was in a binary vector construct, was transferred to tobacco plants via incubation of tobacco leaf explants by Agrobacterium containing mentioned construct. Screening of transgenic plants was carried out in culture media containing antibiotics and certain amounts of salts. Transgenic lines were studied for their ability to produce callus, shoots and roots in media containing different amounts of salt. It was concluded that production of shoots is easier than the other parts of plants in salty condition. Polymerase chain reaction (PCR) was carried out to show integration of osmotin gene into transgenic lines. پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16773_0eeaa69b3af10cf2e4d706006b806ea2.pdf لیلی صمدی author بهروز شاهسون بهبودی author text article 2004 per Nickel (Ni) is a phytotoxic metal under specific soil conditions. Plant roots easily take it up and localize in the periplasmic spaces and vacuoles. Excess doses of Nickel can induce cell death in root cells. We used histochemical and cytochemical methods and the TUNEL assay to study the morphology of cell death. In control roots, programmed cell death is observed in three morphogenic regions: Cap cells, epidermal cell and initial cells of central cylinder. The characteristic hallmarks of this normal death are: cytoplasm and nucleus condensation, cell vacuolization and DNA fragmentation. We also used TUNEL assay (TdT- mediated deoxy- Uracil Nick End Labeling) to detect the 3?-OH ends of broken DNA. In control roots, only cap, epidermal and initial cells of central cylinder are TUNEL-positive which shows DNA fragmentation of these cells. In addition to cap, epidermal and initial cells of central cylinder, in middle concentrations of Ni the TUNEL-positive cells were observed in cortex and meristem. This indicates the induction of cell death by Ni in these cells. The morphology of this induced cell death is different from natural cell death, considering apoptotic-bodies. In natural apoptotic death of root tip cells, apoptotic-bodies were not observed.While they were observed in Ni-induced cell death. The fate of apoptotic-bodies in plant cells is not yet well understood. پرديس علوم 1 v. 0 no. 2004 https://jos.ut.ac.ir/article_16774_4e23bc7a011c52f1cc495ac4303e3033.pdf