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The effect of various concentrations of sodium chloride on the interaction between sodium n-dodecyl sulphate (SDS) and Catalases (Aspergillus niger and Bovine liver) were compared by a combination of equilibrium dialysis, electrophoresis and viscometry techniques at pH=3.2, glycine buffer.
A theoretical approach based on the binding potential concept of Wyman is presented and has been used to make estimates of the apparent Gibbs energies of catalases-SDS complexes, comparing the structural stability of said catalases. The binding date have been used in terms of the Hill and Scatchard equations
which it is shown the type of binding.