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When cultured on media containing various amounts of different hormones, plant organ segments can produce neoformed organs. Just like the organs produced from seeds, these neoformed organs can also go on to serve as intact plant organs. In this research, the seeds from three species of Hyoscyamus genus, including H. pusillus, H. arachnoideus, annual H. niger and biennial H. niger from two different geographical regions, were cultured to obtain sterile seedlings. Then, organogenesis (rhizogenesis, caulogenesis, and leaf production) was assessed with the culture of seeds and organ segments of the obtained sterile plants on media containing various amounts of various hormones. Statistical assessments on the results showed us that the best rhizogenesis was obtained with root culture of biennial H. niger, on MS medium containing IAA (0.2 mg/L), and stem culture of biennial H. niger, on MS medium containing IAA (0.2 mg/L) and Kin (1.5 mg/L). Also, the culture of H. pusillus, H. arachnoideus, and annual H. niger species on MS medium containing 2,4-D (0.32 mg/L) showed the highest rhizogenesis. The highest caulogenesis in biennial H. niger species was obtained when the calli obtained from the stem culture on MS medium containing IAA (0.09 mg/L) and BAP (1.12 mg/L), were sub-cultured on MS medium containing BAP (1.12 mg/L) and GA3 (2.5 mg/L). Also the highest caulo-genesis was observed on the calli obtained from the culture of the seeds of H. pusillus on MS medium containing BAP (0.5 mg/L) and NAA (0.5 mg/L). The highest leaf production was observed with the stem culture of H. arachnoideus on MS medium containing Kin (1.5 mg/L), and the culture of H. pusillus seeds on MS medium containing BAP (0.5 mg/L) and NAA (0.5 mg/L).