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Required conditions for neoformation of roots and buds has been studied in several plants (7) and also in some Peperomia species (1,2). In 1964, Harris and his collaborators showed by use of Squares segments of Peperomia Sandersii's leaf in non-sterile condition, that this segments are able to produce roots and buds which the latter is formed in the proximal pole of the roots. In 1968, Bigot and Brulfert, studying the rhizogenesis and caulogenesis on detached leaves of Peperomia griseo-argentea in the non—sterile condition confirmed the results obtained about Peperomia sandersii, and concluded that in the Peperomia, root is necessary for caulogenesis. In 1978, Ebrahimzadeh and Amide, by determining the required hormon conditions for root and bud formation on internodal segments of stem of Peperomia blanda, showed that in the sterile condition, formation of roots and buds were accomplished independendly. So it seemed intresting to study this problem for comparison with results of previous studies by utilization of the square segments of
Peperomia bland's leaf and to clarify the origin of roots and buds.