Experiments were conducted on four species of Quercus (Q. brantii, Q. infectoria, Q. castanifolia and Q. libani). Cotyledon culture using MS medium containing BAP and IAA was applied for callus production. In the medium with BAP (1.0 mg/lit) and IAA (2.0 mg/lit) all species produced callus, however, in this medium callus was produced after 8 weeks, after 12 weeks globular embryogenic nodules were observed on the surface of Q. castaneifolia calli and finally developed somatic embryos and plant regeneration. Stem and leaf culture did not produce any callus. No response was revealed in explants cultured on hormone free medium.