In this research, several soil and wastewater samples from Hexamine producing factory were cultured in complex and minimum medium containing hexamine. Twenty nine bacteria, 2 yeasts and 3 molds were isolated. Nine bacterial and 2 yeast strains grew in medium containing 10000 mg/l hexamine. The bacterial strains degraded more than 50% and yeast strains degraded 100% of Hexamine. After adaptation of strains, 12 strains of bacteria (Micrococcus and Pseudomonas strains), one yeast strain and two mold strains (Alternaria) degraded hexamin in medium containing 50000 mg / l hexamin.
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