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As one of basic requirements for resistance against enviromental stresses, plants produce and accumulate specific proteins inside the cells. Osmotin protein encoded by osmotin gene was reported to cause resistance to pathogens and environmental stresses such as salt-stress. Stress genetic engineered osmotin gene which was in a binary vector construct, was transferred to tobacco plants via incubation of tobacco leaf explants by Agrobacterium containing mentioned construct. Screening of transgenic plants was carried out in culture media containing antibiotics and certain amounts of salts. Transgenic lines were studied for their ability to produce callus, shoots and roots in media containing different amounts of salt. It was concluded that production of shoots is easier than the other parts of plants in salty condition. Polymerase chain reaction (PCR) was carried out to show integration of osmotin gene into transgenic lines.